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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was identified to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two additional splice variants ended up noted but in-depth nucleotide sequence data isn't nonetheless obtainable.
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Blocking peptides are peptides that bind specially to the focus on antibody and block antibody binding. These peptide ordinarily incorporates the epitope recognized through the antibody.
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This guidebook outlines further attainable optimization of cellular and immunoassay parameters to be certain the absolute best outcomes are...
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the absolute best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Goal Facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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